Detection kit for Monkeypox virus is designed to detect DNA from Monkeypox virus in specimens such as serum or plasma,
vesicular or pustular lesion fluid collected from individuals.
Monkeypox virus ( MPV) is a double- stranded DNA, zoonotic virus and a species of the genus Orthopox virus in the family Poxviridae,
and causes a human disease that resembles smallpox.
Positive test results are indicative of the presence of Monkeypox virus DNA; whereas clinical correlation with patient history and other
diagnostic information is necessary for the determination of patient infection status. Positive results from this test do not rule out bacterial
infection or co- infection with other viruses.
Negative test results from the test do not completely preclude Monkeypox virus infection and should not be used as the sole basis for
patient management decisions. Negative results must be used in combination with clinical observations, patient history, and epidemiological information for a medical decision.
Experimental operators should have received professional training in gene amplification or molecular biology method testing, and have
relevant experimental operating qualifications. The laboratory should have reasonable biological safety precautions and protective procedures.
Storage Conditions and Validity
This kit shall be stored at the temperature - 2 0 ± 5 ℃ in dark and avoid repeated freezing and thawing ( if freezing and thawing , must not exceed
4 times), with a validity period of 12 months . It can be stored at 2~8 ℃ after opening the cover, with a validity period of 30 days .
The kit shall be transported in an ice box .
Test Method
1. Nucleic acid extraction
2. PCR reagent preparation
3. Add samples
4. PCR amplification
5. QC standard
6. Result interpretation
